Survey of transposable elements in sugarcane expressed sequence tags (ESTs)
نویسندگان
چکیده
منابع مشابه
Preliminary analysis of microsatellite markers derived from sugarcane expressed sequence tags (ESTs)
Expressed sequence tags (ESTs) in the sugarcane (Saccharum spp) database (SUCEST) were electronically searched and 402 microsatellites identified. Various dinucleotide and trinucleotide simple sequence repeat (SSR) motifs were found, with these being more frequently observed in ESTs obtained from flower cDNA libraries. PCR primers were designed for 20 of these SSRs and were tested on eight suga...
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We present evidence of cross-hybridization artifact intrinsic to spotted single-dye cDNA microarrays as a result of cDNA containing 5’-end sequences of consecutive thymidine (dT) residues. These poly(dT) tracts result from the synthesis, via oligo (dT) primed reverse transcription, of expressed sequence tags (EST) cDNA from a polyadenylated mRNA template. Analysis of gene expression data from t...
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ژورنال
عنوان ژورنال: Genetics and Molecular Biology
سال: 2001
ISSN: 1415-4757
DOI: 10.1590/s1415-47572001000100020